COMPARATIVE STUDIES ON THE DIAGNOSIS OF SALMONELLA TYPHI USING RAPID KIT AND WIDAL

COMPARATIVE STUDIES ON THE DIAGNOSIS OF SALMONELLA TYPHI USING RAPID KIT AND WIDAL

ABSTRACT

Typhoid fever continues to be a public health issue in most developing countries.Typhoid Fever ranks among the first twenty causes of outpatient illness. Among the numerous challenges in addressing the disease burden of Typhoid is the quest to secure a more reliable and standardized laboratory diagnoses of Salmonella infections to be able to win the surveillance battle on Typhoid fever. The purpose of this study was to compare the specificity and sensitivity of Typhidot and Widal serological tests for the detection of typhoid fever in Cameroon. A prospective hospital based longitudinal study was conducted by gathering samples of 292 potential patients who showed features of Typhoid infection. Adopting a purposive sampling technique, patients who met the inclusion criteria were conveniently sampled and enrolled. Blood culture was used as the standard protocol after which sensitivity, specificity and positive predictive values were compared between Typhidot and Widal serological test kits. Data was analyzed using SPSS version 20 to process after which results were presented descriptively. Chi square test of association was performed for categorical variables .The study on 292 individuals recorded sensitivity of widal (95%) and typhidot (85.8%) with the specificity of widal (54.0%) and Typhidot (90.1%). This observation indicates Widal test is much more sensitive than the typhidot in the study area. The study established that both tests could be equally, sensitive and specific in diagnosing typhoid since they both had an  equal accuracy (98.0) making them suitable for rapid diagnosis. The study found high detection of S. typhi by blood culture diagnosis, with S. paratyphi and S. typhimurun occurring least. This study has implication for further study to be carried out for confirmatory purposes as well as to determine prevalence of infection and antigenic variants that were not captured in the present study.

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MICROBIAL CONTAMINATION OF WELL WATER WITHIN MAKURDI METEROPOLIS

MICROBIAL CONTAMINATION OF WELL WATER WITHIN MAKURDI METEROPOLIS

CHAPTER ONE/INTRODUCTION

Background to the Study

The inadequate supply of clean drinkable waters and the frequent pollution of existing supplies create very grave health problems for people in developing countries like Nigeria. Water serves as a vehicle for the transmission of diseases like typhoid fever, cholera, rashes, diarrhea, dysentery, Gastro-enteritis etc which have contributed immensely to the stagnation of the economic development of some of these nations (Okoufu et al., 1990).

 

In Nigeria, several studies have been carried out in urban areas on ground water contamination (Awalla, 2002; Akpabio and Ebong, 2004; Egbulem, 2003; Ahmed, 2003; Adebayo and Bashire, 2002). The uniform findings from these studies is that groundwater is highly contaminated and clinically unsafe for human consumption. With increasing population densities of urban areas and the corresponding increase in demand for social amenities, it has become increasingly difficult to meet all the water requirements in quantity, regularity and quality. The public water supply is generally inadequate and in most cases inaccessible, the supply is intermittent and unreliable thus resulting into high degree of population densities and concentration of socio-economic activities, it has become increasingly difficult to meet all the water requirements.

 

The public water supply is generally inadequate and in most cases inaccessible, the supply is intermittent and unreliable, thus resulting into high dependency on unsafe supplementary sources such as streams, hand dug wells and ponds (Kakulu and Osibanjo, 1992; Olajire and Imeppeoria, 2001; Nnodu and Ilo, 2002; Owuama and Uzoije, 2005; Ocheri, 2006; Maxwell et al., 2010). Concern over the quality of water harnessed especially from the hand dug wells have received wide attention among researchers (Ovrawah and Hymore, 2001; Ehinola and Coker, 2002; Nnodu and Ilo, 2002; Ogunbadewa, 2002; Omofonmwam and Eseigbe, 2009). Consistent in their findings is that water from hand dug wells are polluted through physical processes, geochemistry of the environment and anthropogenic activities. Consequently consumers of such waters are exposed to series of health risks. This study investigated the bacteriological characteristics of well water in the study area.

Statement of the Problem

Water has been recognized as a potential carrier of diseases and in line with frequent outbreak of typhoid fever, cholera and other water borne diseases; it is pertinent that water be adequately checked and possibly treated particularly well water which is the focus of this work.

Aim and Objectives of the Study

The main objective of this study is to investigate the Microbial Contamination Of Well Water Within Makurdi Meteropolis

The study made up of the following specific objectives;

  1. To investigate the bacteria commonly present in well water in Makurdi
  2. To find out the extent of microbial contamination in well water in different areas of Makurdi
  3. To identify the source of the microbial contamination of well water within Makurdi Metropolis.

Scope of the Study

The study is limited to Makurdi metropolis.Both physico-chemical and bacteriological parameters are considered. The physico-chemical parameters considered are; Turbidity, Potential Hydrogen (pH), Total Dissolved Solid, Iron, Colour, Conductivity, Odour, Chloride, Fluorides and Nitrate while the bacteriological parameters include: Coli- form and Escherichia Coli (E-Coli).

Significance of the Study

The study is significant because it will aid in policy formulation by relevant government agencies and institutions in the formulation of policies regarding water production and distribution in Makurdi metropolis. The study will be of immense importance to members of the public as it can go a long way in enlightening them on the dangers of drinking unsafe and unhygienic water without treatment. The study will act as a guide to scholars and researchers who may wish to carry out research in a similar area especially as regards water quality.

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Bacteriological and physiochemical of food spices

Bacteriological and physio-chemical of food spices

ABSTRACT

There has been constant an increasing the search alternative and efficient compounds for food conservation, aiming a partial or total replacement of antimicrobial chemical additives. Spices offer a promising alternative for food safety. Inhibitory activity of spices and derivatives on the growth of bacteria, yeasts, fungi and microbial toxins synthesis has been well reported, so they could be used in food conservation as main or as adjuvant antimicrobial compounds in order to assure the production of microbiologically stable foods.

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The potential of actinomycetes and fungi to degrade spent oil from motor mechanic site soil

The potential of actinomycetes and fungi to degrade spent oil from motor mechanic site soil

Abstract and Figures

ARTICLE INFO ABSTRACT The degradation potentials of bacteria and actinomycetes of spent motorcycle lubricating oil were investigated using standard microbiological procedures. Ten composite soil samples were collected from ten different motorcycle mechanic workshops in Benin City. The mean heterotrophic count for bacteria and actinomycetes ranged from 68 x 10 4 cfu/g to 155 x 10 4 cfu/g and 43 x 10 4 cfu/g to 85 x 10 4 cfu/g respectively. The mean hydrocarbon degrading bacteria counted ranged from 47 x 10 4 cfu/g to 89 x 10 4 cfu/g, while that of actinomycetes ranged from 18 x 10 4 cfu/g to 46 x 10 4 cfu/g, indicating that the bacteria was greater numerically than the actinomycetes. The preliminary screening test carried out showed that the predominant hydrocarbon degrading bacteria belonged to Micrococcus and Sporosarcina species while that of actinomycetes were Nocardia sp , Gordonia sp , Micromonospora sp and Rhodococcus sp. Biodegradation test conducted revealed a range of 2.5% to 6.6% degradation and 1.035% to 7.53% degradation of the spent lubricating oil in bacteria and actinomycetes respectively, indicating a higher degradation of the spent oil by the actinomycetes.

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